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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA011846LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA011846LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA011846LA01HU at dilution of 1:100

ISL1 Antibody

CSB-PA011846LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetISL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ISL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3670
  • Target name
    ISL1
  • Target description
    ISL LIM homeobox 1
  • Target synonyms
    insulin gene enhancer protein ISL-1; Isl-1; ISLET1; islet-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61371
  • Protein Name
    Insulin gene enhancer protein ISL-1
  • Scientific Description
    Binds and regulates the promoters of the insulin, glucagon and somatostatin genes. Involved in the specificarion of motor neurons in cooperation with LHX3 and LDB1 (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: Hela cell lysates probed with Islet-1 Polyclonal Antibody, Unconjugated (bs-7346R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Islet 1 using anti-Islet 1 antibody (A02969-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: PC12 whole cell lysates, lane 2: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Islet 1 antigen affinity purified polyclonal antibody (Catalog # A02969-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Islet 1 at approximately 39KD. The expected band size for Islet 1 is at 39KD.
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