Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody [HL2807] (GTX639917) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![Islet 1 antibody [HL2807] detects Islet 1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E13.5 embryo. Green: Islet 1 stained by Islet 1 antibody [HL2807] (GTX639917) diluted at 1:100. Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639917/GTX639917_T-45341_20240419_IHC-P_24052202_181.webp "Islet 1 antibody [HL2807] detects Islet 1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E13.5 embryo. Green: Islet 1 stained by Islet 1 antibody [HL2807] (GTX639917) diluted at 1:100. Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Islet 1 antibody [HL2807] detects Islet 1 protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Islet 1 stained by Islet 1 antibody [HL2807] (GTX639917) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639917/GTX639917_45411_20240621_ICC_IF_24070822_643.webp "Islet 1 antibody [HL2807] detects Islet 1 protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Islet 1 stained by Islet 1 antibody [HL2807] (GTX639917) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody [HL2807] (GTX639917) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Islet 1 antibody [HL2807]
GTX639917
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetISL1
Overview
- SupplierGeneTex
- Product NameIslet 1 antibody [HL2807]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2807
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3670
- Target nameISL1
- Target descriptionISL LIM homeobox 1
- Target synonymsISLET1, Isl-1, insulin gene enhancer protein ISL-1, islet-1
- HostRabbit
- IsotypeIgG
- Protein IDP61371
- Protein NameInsulin gene enhancer protein ISL-1
- Scientific DescriptionThis gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![ICC/IF analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using GTX83120 Islet 1 antibody [1B1]. Green : Islet 1 Blue: DRAQ5 fluorescent DNA dye](https://www.genetex.com/upload/website/prouct_img/normal/GTX83120/GTX83120_20170912_ICCIF_w_23061322_518.webp)
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