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WB analysis of various sample lysates using GTX66565 IST1 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66565 IST1 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66565 IST1 antibody. Dilution : 1:3000 Loading : 25microg per lane

IST1 antibody

GTX66565
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetIST1
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Overview

  • Supplier
    GeneTex
  • Product Name
    IST1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9798
  • Target name
    IST1
  • Target description
    IST1 factor associated with ESCRT-III
  • Target synonyms
    CHMP8; increased sodium tolerance 1 homolog; IST1 homolog; IST1, endosomal sorting complex required for transport-III component; IST1, ESCRT-III associated factor; OLC1; overexpressed in lung cancer 1; putative MAPK-activating protein PM28
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53990
  • Protein Name
    IST1 homolog
  • Scientific Description
    This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HIST1/IST1 using anti-HIST1/IST1 antibody (A05507-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MRC-5 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1/IST1 antigen affinity purified polyclonal antibody (Catalog # A05507-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1/IST1 at approximately 40 kDa. The expected band size for HIST1/IST1 is at 40 kDa.
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