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ProductsBack/ITCH (N1) Antibody, Rabbit Polyclonal

ITCH (N1) Antibody, Rabbit Polyclonal

ORB67190
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
TargetITCH
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Overview

  • Supplier
    Biorbyt
  • Product Name
    ITCH antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB:1:500-1:1000 WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83737
  • Target name
    ITCH
  • Target description
    itchy E3 ubiquitin protein ligase
  • Target synonyms
    ADMFD; AIF4; AIP4; atrophin-1 interacting protein 4; E3 ubiquitin-protein ligase Itchy homolog; HECT-type E3 ubiquitin transferase Itchy homolog; itchy E3 ubiquitin protein ligase homolog; NAPP1; NFE2-associated polypeptide 1
  • Host
    Rabbit
  • Protein IDQ96J02
  • Protein Name
    E3 ubiquitin-protein ligase Itchy homolog
  • Scientific Description
    Rabbit polyclonal to ITCH.E3 ubiquitin-protein ligase Itchy homolog (ITCH) acts as an E3 ubiquitin-protein ligase. ITCHubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It isinvolved in the negative regulation of MAVS-dependent cellular antiviral responses. ITCH is involvedin the regulation of apoptosis and reactive oxygen species levels through the ubiquitination andproteasomal degradation of TXNIP. It mediates the antiapoptotic activity of epidermal growth factorthrough the ubiquitination and proteasomal degradation of p15 BID. Defects in ITCH are the cause ofsyndromic multisystem autoimmune disease (SMAD).
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ITCH using anti-ITCH antibody (A00195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITCH antigen affinity purified polyclonal antibody (Catalog # A00195-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITCH at approximately 103KD. The expected band size for ITCH is at 103KD.
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