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ProductsBack/Mouse anti Integrin alpha 3A

Mouse anti Integrin alpha 3A

MUB0900P
Nordic-MUbio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetITGA3
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Integrin alpha 3A
  • Delivery Days Customer
    7
  • Application Supplier Note
    29A3 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    Immunocytochemistry;Immunohistochemistry (frozen);Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    29A3
  • Gene ID3675
  • Target name
    ITGA3
  • Target description
    integrin subunit alpha 3
  • Target synonyms
    alpha 3 subunit of VLA-3 receptor; antigen CD49C; antigen identified by monoclonal antibody J143; CD49 antigen-like family member C; CD49C; FRP-2; galactoprotein B3; GAPB3; GAP-B3; ILNEB; integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); MSK18; testicular tissue protein Li 96; testicular tissue protein Li 98; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA3a
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP26006
  • Protein Name
    Integrin alpha-3
  • Source
    29A3 is a Mouse monoclonal IgG1, kappa antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a synthetic peptide corresponding to the cytoplasmic domain of the integrin subunit alpha3A including ...
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 3/ITGA3 using anti-Integrin Alpha 3/ITGA3 antibody (A02902-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 3/ITGA3 antigen affinity purified polyclonal antibody (Catalog # A02902-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 3/ITGA3 at approximately 130 kDa. The expected band size for Integrin Alpha 3/ITGA3 is at 130 kDa.
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