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ProductsBack/ITGA3 / CD49c Antibody (Preservative Free)

ITGA3 / CD49c Antibody (Preservative Free)

LS-C815761
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetITGA3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGA3 / CD49c Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3675
  • Target name
    ITGA3
  • Target description
    integrin subunit alpha 3
  • Target synonyms
    CD49C, FRP-2, GAP-B3, GAPB3, ILNEB, JEB7, MSK18, VCA-2, VL3A, VLA3a, integrin alpha-3, CD49 antigen-like family member C, alpha 3 subunit of VLA-3 receptor, antigen CD49C, antigen identified by monoclonal antibody J143, galactoprotein B3, integrin alpha 3, integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor), testicular tissue protein Li 96, testicular tissue protein Li 98, very late activation protein 3 receptor, alpha-3 subunit, very late activation receptor subunit alpha-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 3/ITGA3 using anti-Integrin Alpha 3/ITGA3 antibody (A02902-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 3/ITGA3 antigen affinity purified polyclonal antibody (Catalog # A02902-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 3/ITGA3 at approximately 130 kDa. The expected band size for Integrin Alpha 3/ITGA3 is at 130 kDa.
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