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ITGA3 Polyclonal Antibody

RD218119A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetITGA3
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    ITGA3 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.32 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3675
  • Target name
    ITGA3
  • Target description
    integrin subunit alpha 3
  • Target synonyms
    alpha 3 subunit of VLA-3 receptor; antigen CD49C; antigen identified by monoclonal antibody J143; CD49 antigen-like family member C; CD49C; FRP-2; galactoprotein B3; GAPB3; GAP-B3; ILNEB; integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); MSK18; testicular tissue protein Li 96; testicular tissue protein Li 98; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA3a
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    ITGA3,also named as MSK18,CD49c,GAPB3 and FRP-2,belongs to the integrin alpha chain family. It is a receptor for fibronectin,laminin,collagen,epiligrin,thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 3/ITGA3 using anti-Integrin Alpha 3/ITGA3 antibody (A02902-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 3/ITGA3 antigen affinity purified polyclonal antibody (Catalog # A02902-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 3/ITGA3 at approximately 130 kDa. The expected band size for Integrin Alpha 3/ITGA3 is at 130 kDa.
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