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ProductsBack/ITGB3 / Integrin Beta 3 / CD61 Antibody

ITGB3 / Integrin Beta 3 / CD61 Antibody

LS-C404078
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetITGB3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGB3 / Integrin Beta 3 / CD61 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3690
  • Target name
    ITGB3
  • Target description
    integrin subunit beta 3
  • Target synonyms
    BDPLT16, BDPLT2, BDPLT24, CD61, FMAIT1, GP3A, GPIIIa, GT, GT2, integrin beta-3, antigen CD61, integrin beta 3, integrin beta chain, beta 3, integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61), platelet membrane glycoprotein IIIa
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Integrin beta 3/ITGB3 using anti-Integrin beta 3/ITGB3 antibody (A00587-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 3/ITGB3 antigen affinity purified polyclonal antibody (Catalog # A00587-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin beta 3/ITGB3 at approximately 100 kDa. The expected band size for Integrin beta 3/ITGB3 is at 87 kDa.
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