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ProductsBack/ITGB4 / Integrin Beta 4 Antibody (phospho-Y1510)

ITGB4 / Integrin Beta 4 Antibody (phospho-Y1510)

LS-C368743
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetITGB4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGB4 / Integrin Beta 4 Antibody (phospho-Y1510)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100 - 1:200), WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3691
  • Target name
    ITGB4
  • Target description
    integrin subunit beta 4
  • Target synonyms
    CD104; CD104 antigen; GP150; integrin beta-4
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (A01015-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat ovary tissue lysates, Lane 8: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 4/ITGB4 antigen affinity purified polyclonal antibody (Catalog # A01015-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin beta 4/ITGB4 at approximately 210KD. The expected band size for Integrin beta 4/ITGB4 is at 202KD.
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