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ProductsBack/ITK antibody [N2C1], Internal
ITK antibody [N2C1], Internal detects ITK protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK stained by ITK antibody [N2C1], Internal (GTX113217) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
ITK antibody [N2C1], Internal detects ITK protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK stained by ITK antibody [N2C1], Internal (GTX113217) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
ITK antibody [N2C1], Internal detects ITK protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK stained by ITK antibody [N2C1], Internal (GTX113217) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).

ITK antibody [N2C1], Internal

GTX113217
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetITK
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Overview

  • Supplier
    GeneTex
  • Product Name
    ITK antibody [N2C1], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.25 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3702
  • Target name
    ITK
  • Target description
    IL2 inducible T cell kinase
  • Target synonyms
    EMT, LPFS1, LYK, PSCTK2, tyrosine-protein kinase ITK/TSK, IL-2-inducible T-cell kinase, T-cell-specific kinase, homolog of mouse T-cell itk/tsk, interleukin-2-inducible T-cell kinase, kinase EMT, tyrosine-protein kinase LYK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08881
  • Protein Name
    Tyrosine-protein kinase ITK/TSK
  • Scientific Description
    This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of ITK using anti-ITK antibody (A01385-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITK antigen affinity purified polyclonal antibody (Catalog # A01385-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITK at approximately 72 kDa. The expected band size for ITK is at 72 kDa.
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