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ProductsBack/ITK Mouse Monoclonal Antibody [Clone ID: 5G12C4]
Western blot analysis using ITK antibody Cat.-No AM06236SU-N against truncated Trx-ITK recombinant protein (Lane 1).
Western blot analysis using ITK antibody Cat.-No AM06236SU-N against truncated Trx-ITK recombinant protein (Lane 1).
Western blot analysis using ITK antibody Cat.-No AM06236SU-N against truncated Trx-ITK recombinant protein (Lane 1).

ITK Mouse Monoclonal Antibody [Clone ID: 5G12C4]

AM06236SU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetITK
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    ITK Mouse Monoclonal Antibody [Clone ID: 5G12C4]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5G12C4
  • Gene ID3702
  • Target name
    ITK
  • Target description
    IL2 inducible T cell kinase
  • Target synonyms
    EMT, LPFS1, LYK, PSCTK2, tyrosine-protein kinase ITK/TSK, IL-2-inducible T-cell kinase, T-cell-specific kinase, homolog of mouse T-cell itk/tsk, interleukin-2-inducible T-cell kinase, kinase EMT, tyrosine-protein kinase LYK
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ08881
  • Protein Name
    Tyrosine-protein kinase ITK/TSK
  • Scientific Description
    ITK mouse monoclonal antibody, clone 5G12C4, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of ITK using anti-ITK antibody (A01385-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITK antigen affinity purified polyclonal antibody (Catalog # A01385-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITK at approximately 72 kDa. The expected band size for ITK is at 72 kDa.
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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N3C3] (GTX113215) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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