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ProductsBack/ITSN1 Antibody
Western Blot Positive WB detected in: 293T whole cell lysate All lanes: ITSN1 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 196, 138, 130, 188, 116, 195, 189, 134, 127, 126, 105 kDa Observed band size: 196 kDa
Western Blot Positive WB detected in: 293T whole cell lysate All lanes: ITSN1 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 196, 138, 130, 188, 116, 195, 189, 134, 127, 126, 105 kDa Observed band size: 196 kDa
Western Blot Positive WB detected in: 293T whole cell lysate All lanes: ITSN1 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 196, 138, 130, 188, 116, 195, 189, 134, 127, 126, 105 kDa Observed band size: 196 kDa

ITSN1 Antibody

CSB-PA621885LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetITSN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ITSN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6453
  • Target name
    ITSN1
  • Target description
    intersectin 1
  • Target synonyms
    ITSN, SH3D1A, SH3P17, intersectin-1, SH3 domain-containing protein 1A, Src homology 3 domain-containing protein, human intersectin-SH3 domain-containing protein SH3P17, intersectin 1 (SH3 domain protein)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15811
  • Protein Name
    Intersectin-1
  • Scientific Description
    Acts as guanine nucleotide exchange factor (GEF) specific for the CDC42 GTPase (By similarity). Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Intersectin 1/ITSN1 using anti-Intersectin 1/ITSN1 antibody (A03616-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Intersectin 1/ITSN1 antigen affinity purified polyclonal antibody (Catalog # A03616-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Intersectin 1/ITSN1 at approximately 150 kDa. The expected band size for Intersectin 1/ITSN1 is at 195 kDa.
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