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ITSN1 Polyclonal Antibody

CAC15659
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetITSN1
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Overview

  • Supplier
    Biomatik
  • Product Name
    ITSN1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6453
  • Target name
    ITSN1
  • Target description
    intersectin 1
  • Target synonyms
    ITSN, SH3D1A, SH3P17, intersectin-1, SH3 domain-containing protein 1A, Src homology 3 domain-containing protein, human intersectin-SH3 domain-containing protein SH3P17, intersectin 1 (SH3 domain protein)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15811
  • Protein Name
    Intersectin-1
  • Scientific Description
    The ITSN1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Intersectin 1/ITSN1 using anti-Intersectin 1/ITSN1 antibody (A03616-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Intersectin 1/ITSN1 antigen affinity purified polyclonal antibody (Catalog # A03616-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Intersectin 1/ITSN1 at approximately 150 kDa. The expected band size for Intersectin 1/ITSN1 is at 195 kDa.
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