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Western Blot analysis of HuvEc cells using Involucrin Polyclonal Antibody
Western Blot analysis of HuvEc cells using Involucrin Polyclonal Antibody
Western Blot analysis of HuvEc cells using Involucrin Polyclonal Antibody

IVL Antibody

CSB-PA003053
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIVL
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Overview

  • Supplier
    Cusabio
  • Product Name
    IVL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3713
  • Target name
    IVL
  • Target description
    involucrin
  • Target synonyms
    involucrin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07476
  • Protein Name
    Involucrin
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Involucrin/IVL using anti-Involucrin/IVL antibody (PB9712). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Involucrin/IVL antigen affinity purified polyclonal antibody (Catalog # PB9712) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Involucrin/IVL at approximately 120 kDa. The expected band size for Involucrin/IVL is at 68 kDa.
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