Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Involucrin(GTX116012) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (GTX116012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116012/GTX116012_40177_20151112_WB_B_w_23060519_582.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (GTX116012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (GTX116012) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116012/GTX116012_42340_20181026_WB_w_23060519_793.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (GTX116012) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Involucrin(GTX116012) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Involucrin antibody [N1N2], N-term
GTX116012
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIVL
Overview
- SupplierGeneTex
- Product NameInvolucrin antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.72 mg/ml
- ConjugateUnconjugated
- Gene ID3713
- Target nameIVL
- Target descriptioninvolucrin
- Target synonymsinvolucrin
- HostRabbit
- IsotypeIgG
- Protein IDP07476
- Protein NameInvolucrin
- Scientific DescriptionInvolucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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