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Western blot All lanes: IWS1 antibody at 3.84microg/ml Lane 1: 293T whole cell lysate Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 92, 57, 70 kDa Observed band size: 70 kDa
Western blot All lanes: IWS1 antibody at 3.84microg/ml Lane 1: 293T whole cell lysate Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 92, 57, 70 kDa Observed band size: 70 kDa
Western blot All lanes: IWS1 antibody at 3.84microg/ml Lane 1: 293T whole cell lysate Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 92, 57, 70 kDa Observed band size: 70 kDa

IWS1 Antibody

CSB-PA822304ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIWS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    IWS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55677
  • Target name
    IWS1
  • Target description
    interacts with SUPT6H, CTD assembly factor 1
  • Target synonyms
    interacts with Spt6; IWS1 homolog; IWS1, SUPT6H interacting protein; IWS1-like protein; protein IWS1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96ST2
  • Protein Name
    Protein IWS1 homolog
  • Scientific Description
    Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IWS1 using anti-IWS1 antibody (A05958-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IWS1 antigen affinity purified polyclonal antibody (Catalog # A05958-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IWS1 at approximately 140 kDa. The expected band size for IWS1 is at 140 kDa.
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