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Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells.
Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells.
Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells.

Anti-IWS1 Antibody

HPA035719
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetIWS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IWS1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55677
  • Target name
    IWS1
  • Target description
    interacts with SUPT6H, CTD assembly factor 1
  • Target synonyms
    interacts with Spt6; IWS1 homolog; IWS1, SUPT6H interacting protein; IWS1-like protein; protein IWS1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96ST2
  • Protein Name
    Protein IWS1 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IWS1 using anti-IWS1 antibody (A05958-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IWS1 antigen affinity purified polyclonal antibody (Catalog # A05958-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IWS1 at approximately 140 kDa. The expected band size for IWS1 is at 140 kDa.
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