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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA049990(JAG2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA049990(JAG2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA049990(JAG2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

JAG2 Antibody

CSB-PA049990
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetJAG2
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Overview

  • Supplier
    Cusabio
  • Product Name
    JAG2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3714
  • Target name
    JAG2
  • Target description
    jagged canonical Notch ligand 2
  • Target synonyms
    HJ2; jagged 2; protein jagged-2; SER2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y219
  • Protein Name
    Protein jagged-2
  • Scientific Description
    The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for this gene.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Jagged 2/JAG2 using anti-Jagged 2/JAG2 antibody (A01428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Jagged 2/JAG2 antigen affinity purified polyclonal antibody (Catalog # A01428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Jagged 2/JAG2 at approximately 130 kDa. The expected band size for Jagged 2/JAG2 is at 133 kDa.
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