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Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.

Anti-JAG2 Antibody

HPA030636
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetJAG2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-JAG2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3714
  • Target name
    JAG2
  • Target description
    jagged canonical Notch ligand 2
  • Target synonyms
    HJ2, LGMDR27, SER2, protein jagged-2, jagged 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y219
  • Protein Name
    Protein jagged-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Jagged 2/JAG2 using anti-Jagged 2/JAG2 antibody (A01428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Jagged 2/JAG2 antigen affinity purified polyclonal antibody (Catalog # A01428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Jagged 2/JAG2 at approximately 130 kDa. The expected band size for Jagged 2/JAG2 is at 133 kDa.
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