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ProductsBack/Jagged 1 antibody [4A24]

Jagged 1 antibody [4A24]

GTX52879
GeneTex
ApplicationsFlow Cytometry, Western Blot, Neutralisation/Blocking
DownloadDatasheet
Product group Antibodies
TargetJAG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Jagged 1 antibody [4A24]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. FACS: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4A24
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID182
  • Target name
    JAG1
  • Target description
    jagged canonical Notch ligand 1
  • Target synonyms
    AGS; AGS1; AHD; AWS; CD339; DCHE; HJ1; JAGL1; protein jagged-1
  • Host
    Mouse
  • Isotype
    IgG2
  • Protein IDP78504
  • Protein Name
    Protein jagged-1
  • Scientific Description
    The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Acute Lymphoblastic Leukemia Cells Inhibit the Differentiation of Bone Mesenchymal Stem Cells into Osteoblasts In Vitro by Activating Notch Signaling. Yang GC et al., 2015, Stem Cells Int
    Read more

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Figure 1. Western blot analysis of Jagged1/JAG1 using anti-Jagged1/JAG1 antibody (A00640-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Jagged1/JAG1 antigen affinity purified polyclonal antibody (Catalog # A00640-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Jagged1/JAG1 at approximately 180 kDa. The expected band size for Jagged1/JAG1 is at 134 kDa.
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