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ProductsBack/Jagged 1 antibody [4A24]

Jagged 1 antibody [4A24]

GTX52879
GeneTex
ApplicationsFlow Cytometry, Western Blot, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman
TargetJAG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Jagged 1 antibody [4A24]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. FACS: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4A24
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID182
  • Target name
    JAG1
  • Target description
    jagged canonical Notch ligand 1
  • Target synonyms
    AGS, AGS1, AHD, AWS, CD339, CMT2HH, DCHE, HJ1, JAGL1, protein jagged-1
  • Host
    Mouse
  • Isotype
    IgG2
  • Protein IDP78504
  • Protein Name
    Protein jagged-1
  • Scientific Description
    The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yang GC, Xu YH, Chen HX, et al. Acute Lymphoblastic Leukemia Cells Inhibit the Differentiation of Bone Mesenchymal Stem Cells into Osteoblasts In Vitro by Activating Notch Signaling. Stem Cells Int. 2015,2015:162410. doi: 10.1155/2015/162410
    Read this paper

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Figure 1. Western blot analysis of Jagged1/JAG1 using anti-Jagged1/JAG1 antibody (A00640-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Jagged1/JAG1 antigen affinity purified polyclonal antibody (Catalog # A00640-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Jagged1/JAG1 at approximately 180 kDa. The expected band size for Jagged1/JAG1 is at 134 kDa.
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