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Jagged 2 Antibody (YA2941)

HY-P83196
MedChem Express
TargetJAG2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Jagged 2 Antibody (YA2941)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3714
  • Target name
    JAG2
  • Target description
    jagged canonical Notch ligand 2
  • Target synonyms
    HJ2; jagged 2; protein jagged-2; SER2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Jagged 2 Antibody (YA2941) is a non-conjugated IgG antibody, targeting Jagged 2, with a predicted molecular weight of 133 kDa (observed band size: 145 kDa). Jagged 2 Antibody (YA2941) can be used for WB experiment in human, mouse background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Jagged 2/JAG2 using anti-Jagged 2/JAG2 antibody (A01428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Jagged 2/JAG2 antigen affinity purified polyclonal antibody (Catalog # A01428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Jagged 2/JAG2 at approximately 130 kDa. The expected band size for Jagged 2/JAG2 is at 133 kDa.
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