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ProductsBack/JNK1 (MAPK8) (pT-G/P-pY Motif) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H8]
Immunoblot Analysis Jurkat cells were cultured under serum-free conditions for 48h and subsequently were either unstimulated (lane 1) or stimulated with 300mM sorbit (lane 2), 1mM arsenite (lane 3) or 1mM pervanadate (lane 4).The cell lysates were separated by SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK1/2-9H8 at 0.5ug/ml for 1h at 15-22°C and developed by ECL.
Immunoblot Analysis Jurkat cells were cultured under serum-free conditions for 48h and subsequently were either unstimulated (lane 1) or stimulated with 300mM sorbit (lane 2), 1mM arsenite (lane 3) or 1mM pervanadate (lane 4).The cell lysates were separated by SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK1/2-9H8 at 0.5ug/ml for 1h at 15-22°C and developed by ECL.
Immunoblot Analysis Jurkat cells were cultured under serum-free conditions for 48h and subsequently were either unstimulated (lane 1) or stimulated with 300mM sorbit (lane 2), 1mM arsenite (lane 3) or 1mM pervanadate (lane 4).The cell lysates were separated by SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK1/2-9H8 at 0.5ug/ml for 1h at 15-22°C and developed by ECL.

JNK1 (MAPK8) (pT-G/P-pY Motif) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H8]

AM00082PU-N
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetMAPK8
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    JNK1 (MAPK8) (pT-G/P-pY Motif) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H8]
  • Delivery Days Customer
    14
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9H8
  • Gene ID5599
  • Target name
    MAPK8
  • Target description
    mitogen-activated protein kinase 8
  • Target synonyms
    c-Jun N-terminal kinase 1; JNK; JNK1; JNK1A2; JNK21B1/2; JNK-46; JUN N-terminal kinase; MAP kinase 8; mitogen-activated protein kinase 8; PRKM8; SAPK1; SAPK1c; stress-activated protein kinase 1; stress-activated protein kinase 1c
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP45983
  • Protein Name
    Mitogen-activated protein kinase 8
  • Scientific Description
    JNK1 (MAPK8) (pT-G/P-pY Motif) (incl. pos. control) mouse monoclonal antibody, clone 9H8, Purified
  • Storage Instruction
    -20°C,-80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of JNK1/2/3 using anti-JNK1/2/3 antibody (M02608-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK1/2/3 antigen affinity purified monoclonal antibody (Catalog # M02608-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JNK1/2/3 at approximately 40, 48 kDa. The expected band size for JNK1/2/3 is at 48 kDa.
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