Western blot analysis of extracts of various cell lines, using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 antibody (TA378318) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 180s.
JNK1 (MAPK8) Rabbit Polyclonal Antibody
TA378318
Overview
- SupplierOriGene
- Product NameJNK1 (MAPK8) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID5599
- Target nameMAPK8
- Target descriptionmitogen-activated protein kinase 8
- Target synonymsc-Jun N-terminal kinase 1; JNK; JNK1; JNK1A2; JNK21B1/2; JNK-46; JUN N-terminal kinase; MAP kinase 8; mitogen-activated protein kinase 8; PRKM8; SAPK1; SAPK1c; stress-activated protein kinase 1; stress-activated protein kinase 1c
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPhospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse
- UNSPSC12352203