Western blot analysis of various lysates using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit pAb (TA378318) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Western blot analysis of various lysates using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit pAb (TA378318) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
JNK1 (MAPK8) Rabbit Polyclonal Antibody
TA378318
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetMAPK8
Overview
- SupplierOriGene
- Product NameJNK1 (MAPK8) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID5599
- Target nameMAPK8
- Target descriptionmitogen-activated protein kinase 8
- Target synonymsJNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c, mitogen-activated protein kinase 8, JUN N-terminal kinase, MAP kinase 8, c-Jun N-terminal kinase 1, stress-activated protein kinase 1, stress-activated protein kinase 1c
- HostRabbit
- IsotypeIgG
- Protein IDP45983
- Protein NameMitogen-activated protein kinase 8
- Scientific DescriptionPhospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse
- Storage Instruction-20°C
- UNSPSC12352203
MSDS
Related products
Product group Antibodies
Anti-MAPK8 Antibody144-00288
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAPK8
- SizePrice
Product group Antibodies
MAPK8 Polyclonal AntibodyCAC14610
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetMAPK8
- SizePrice
Product group Antibodies
References
JNK1 + JNK3 Polyclonal AntibodyBS-0501R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
TargetMAPK8
- SizePrice
Product group Antibodies
Anti-JNK1 AntibodyA29715
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
- SizePrice
Product group Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetMAPK8
- SizePrice
Product group Antibodies
MAPK8/MAPK9/MAPK10 AntibodyCSB-PA003084
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK8
- SizePrice
Product group Antibodies
ApplicationsELISA
ReactivityHuman
TargetMAPK8
- SizePrice