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ProductsBack/JUP/CTNNG/Junction Plakoglobin Antibody (HRP)

JUP/CTNNG/Junction Plakoglobin Antibody (HRP)

LS-C544021
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetJUP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    JUP/CTNNG/Junction Plakoglobin Antibody (HRP)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IF, IP, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID3728
  • Target name
    JUP
  • Target description
    junction plakoglobin
  • Target synonyms
    CTNNG, DP3, DPIII, PDGB, PG, PKGB, junction plakoglobin, catenin (cadherin-associated protein), gamma 80kDa, desmoplakin III, desmoplakin-3, desmosomal protein 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates, Lane 4: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Catenin antigen affinity purified polyclonal antibody (Catalog # PB9185) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for gamma Catenin at approximately 82 kDa. The expected band size for gamma Catenin is at 82 kDa.
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