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ProductsBack/KAT5 / TIP60 Antibody

KAT5 / TIP60 Antibody

LS-C748321
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetKAT5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KAT5 / TIP60 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 53kDa/56kDa/58kDa/61kDa, while the observed MW by Western blot was 56kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:20 - 1:50), WB (1:500 - 1:2000) The predicted MW is 53kDa/56kDa/58kDa/61kDa, while the observed MW by Western blot was 56kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10524
  • Target name
    KAT5
  • Target description
    lysine acetyltransferase 5
  • Target synonyms
    ESA1, HTATIP, HTATIP1, NEDFASB, PLIP, TIP, TIP60, ZC2HC5, cPLA2, histone acetyltransferase KAT5, 60 kDa Tat-interactive protein, HIV-1 Tat interactive protein, 60kDa, K(lysine) acetyltransferase 5, K-acetyltransferase 5, Tat interacting protein, 60kDa, cPLA(2)-interacting protein, cPLA2 interacting protein, histone acetyltransferase HTATIP, protein 2-hydroxyisobutyryltransferase KAT5, protein acetyltransferase KAT5, protein crotonyltransferase KAT5, protein lactyltransferase KAT5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tip60/KAT5 using anti-Tip60/KAT5 antibody (A01393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tip60/KAT5 antigen affinity purified polyclonal antibody (Catalog # A01393-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tip60/KAT5 at approximately 60 kDa. The expected band size for Tip60/KAT5 is at 59 kDa.
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