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Western Blot analysis of HeLa cells using KV1.1 Polyclonal Antibody
Western Blot analysis of HeLa cells using KV1.1 Polyclonal Antibody
Western Blot analysis of HeLa cells using KV1.1 Polyclonal Antibody

KCNA1 Antibody

CSB-PA003117
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3736
  • Target name
    KCNA1
  • Target description
    potassium voltage-gated channel subfamily A member 1
  • Target synonyms
    AEMK, EA1, HBK1, HUK1, KV1.1, MBK1, MK1, RBK1, potassium voltage-gated channel subfamily A member 1, potassium channel, voltage gated shaker related subfamily A, member 1, potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia with myokymia), voltage-gated K(+) channel HuKI, voltage-gated potassium channel HBK1, voltage-gated potassium channel subunit Kv1.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ09470
  • Protein Name
    Potassium voltage-gated channel subfamily A member 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KCNA1 using anti-KCNA1 antibody (A01813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA1 antigen affinity purified polyclonal antibody (Catalog # A01813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA1 at approximately 56KD. The expected band size for KCNA1 is at 56KD.
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