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KCNA1 / Kv1.1 Antibody

LS-C402402
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetKCNA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KCNA1 / Kv1.1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3736
  • Target name
    KCNA1
  • Target description
    potassium voltage-gated channel subfamily A member 1
  • Target synonyms
    AEMK; EA1; HBK1; HUK1; KV1.1; MBK1; MK1; potassium channel, voltage gated shaker related subfamily A, member 1; potassium voltage-gated channel subfamily A member 1; potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia with myokymia); RBK1; voltage-gated K(+) channel HuKI; voltage-gated potassium channel HBK1; voltage-gated potassium channel subunit Kv1.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNA1 using anti-KCNA1 antibody (A01813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA1 antigen affinity purified polyclonal antibody (Catalog # A01813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA1 at approximately 56KD. The expected band size for KCNA1 is at 56KD.
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