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ProductsBack/KCNA4 / Kv1.4 Antibody (aa609-647)

KCNA4 / Kv1.4 Antibody (aa609-647)

LS-C408019
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetKCNA4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KCNA4 / Kv1.4 Antibody (aa609-647)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (0.5 - 1 µg/ml), WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3739
  • Target name
    KCNA4
  • Target description
    potassium voltage-gated channel subfamily A member 4
  • Target synonyms
    HBK4, HK1, HPCN2, HUKII, KCNA4L, KCNA8, KV1.4, MCIDDS, PCN2, potassium voltage-gated channel subfamily A member 4, cardiac potassium channel, fetal skeletal muscle potassium channel, potassium channel 2, potassium channel, voltage gated shaker related subfamily A, member 4, rapidly inactivating potassium channel, shaker-related potassium channel Kv1.4, type A potassium channel, voltage-gated K(+) channel HuKII, voltage-gated potassium channel HBK4, voltage-gated potassium channel HK1, voltage-gated potassium channel subunit Kv1.4
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Kv1.4 using anti-Kv1.4 antibody (PB9807). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: COLO320 Whole Cell Lysate, Lane 3: HT1080 Whole Cell Lysate, Lane 4: PANC Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv1.4 antigen affinity purified polyclonal antibody (Catalog # PB9807) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kv1.4 at approximately 73 kDa. The expected band size for Kv1.4 is at 73 kDa.
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