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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA012035DSR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA012035DSR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA012035DSR2HU at dilution of 1:100

KCNH1 Antibody

CSB-PA012035DSR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetKCNH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNH1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3756
  • Target name
    KCNH1
  • Target description
    potassium voltage-gated channel subfamily H member 1
  • Target synonyms
    EAG, EAG1, K(V)10.1, Kv10.1, TMBTS, ZLS1, h-eag, hEAG, hEAG1, voltage-gated delayed rectifier potassium channel KCNH1, EAG channel 1, ether-a-go-go 1, ether-a-go-go potassium channel 1, ether-a-go-go, Drosophila, homolog of, potassium channel, voltage gated eag related subfamily H, member 1, potassium voltage-gated channel, subfamily H (eag-related), member 1, voltage-gated potassium channel subunit Kv10.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95259
  • Protein Name
    Voltage-gated delayed rectifier potassium channel KCNH1
  • Scientific Description
    Pore-forming (alpha) subunit of a voltage-gated delayed rectifier potassium channel (PubMed:22732247). Channel properties may be modulated by subunit assembly, but not by cyclic nucleotides (By similarity). Mediates IK(NI) current in myoblasts (PubMed:9738473). Involved in the regulation of cell proliferation and differentiation, in particular adipogenic and osteogenic differentiation in bone marrow-derived mesenchymal stem cells (MSCs) (PubMed:23881642).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KCNH1 using anti-KCNH1 antibody (A01036-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 cell lysate, Lane 2: human HepG2 cell lysate, Lane 3: human A549 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNH1 antigen affinity purified polyclonal antibody (Catalog # A01036-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNH1 at approximately 150KD. The expected band size for KCNH1 is at 111KD.
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