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Western Blot analysis of HeLa cells using KIR5.1 Polyclonal Antibody
Western Blot analysis of HeLa cells using KIR5.1 Polyclonal Antibody
Western Blot analysis of HeLa cells using KIR5.1 Polyclonal Antibody

KCNJ16 Antibody

CSB-PA007704
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNJ16
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNJ16 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3773
  • Target name
    KCNJ16
  • Target description
    potassium inwardly rectifying channel subfamily J member 16
  • Target synonyms
    BIR9, HKTD, KIR5.1, inward rectifier potassium channel 16, inward rectifier K(+) channel Kir5.1, inward rectifier K+ channel KIR5.1, potassium channel, inwardly rectifying subfamily J member 16, potassium voltage-gated channel subfamily J member 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NPI9
  • Protein Name
    Inward rectifier potassium channel 16
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KCNJ16 using anti-KCNJ16 antibody (A10858-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: monkey kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNJ16 antigen affinity purified polyclonal antibody (Catalog # A10858-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNJ16 at approximately 48KD. The expected band size for KCNJ16 is at 48KD.
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