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KCNJ16 / Kir5.1 Antibody (360-440 aa)

LS-C381527
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNJ16
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KCNJ16 / Kir5.1 Antibody (360-440 aa)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000), IF (1:200 - 1:1000), IHC (1:100 - 1:300), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3773
  • Target name
    KCNJ16
  • Target description
    potassium inwardly rectifying channel subfamily J member 16
  • Target synonyms
    BIR9, HKTD, KIR5.1, inward rectifier potassium channel 16, inward rectifier K(+) channel Kir5.1, inward rectifier K+ channel KIR5.1, potassium channel, inwardly rectifying subfamily J member 16, potassium voltage-gated channel subfamily J member 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNJ16 using anti-KCNJ16 antibody (A10858-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: monkey kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNJ16 antigen affinity purified polyclonal antibody (Catalog # A10858-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNJ16 at approximately 48KD. The expected band size for KCNJ16 is at 48KD.
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