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ProductsBack/KCNJ8 / Kir6.1 Antibody

KCNJ8 / Kir6.1 Antibody

LS-C497273
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetKCNJ8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KCNJ8 / Kir6.1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 47kDa, while the observed MW by Western blot was 48kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) The predicted MW is 47kDa, while the observed MW by Western blot was 48kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3764
  • Target name
    KCNJ8
  • Target description
    potassium inwardly rectifying channel subfamily J member 8
  • Target synonyms
    KIR6.1, uKATP-1, ATP-sensitive inward rectifier potassium channel 8, inward rectifier K(+) channel Kir6.1, inwardly rectifying potassium channel KIR6.1, potassium channel, inwardly rectifying subfamily J member 8, potassium voltage-gated channel subfamily J member 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kir6.1/KCNJ8 using anti-Kir6.1/KCNJ8 antibody (A04950-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kir6.1/KCNJ8 antigen affinity purified polyclonal antibody (Catalog # A04950-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kir6.1/KCNJ8 at approximately 48KD. The expected band size for Kir6.1/KCNJ8 is at 48KD.
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TargetKCNJ8
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IHC image of CSB-PA613604LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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