NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM3B antibody [C2C3], C-term (GTX116198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KDM3B antibody [C2C3], C-term (GTX116198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116198/GTX116198_40296_20200904_WB_w_23060519_677.webp "Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KDM3B antibody [C2C3], C-term (GTX116198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![KDM3B antibody [C2C3], C-term immunoprecipitates KDM3B protein in IP experiments. IP samples: NT2D1 whole cell extract A. 50 μg NT2D1 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of KDM3B protein by 4 μg KDM3B antibody [C2C3], C-term (GTX116198) 5 % SDS-PAGE The immunoprecipitated KDM3B protein was detected by KDM3B antibody [C2C3], C-term (GTX116198) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX116198/GTX116198_40296_IP_w_23060519_546.webp "KDM3B antibody [C2C3], C-term immunoprecipitates KDM3B protein in IP experiments. IP samples: NT2D1 whole cell extract A. 50 μg NT2D1 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of KDM3B protein by 4 μg KDM3B antibody [C2C3], C-term (GTX116198) 5 % SDS-PAGE The immunoprecipitated KDM3B protein was detected by KDM3B antibody [C2C3], C-term (GTX116198) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM3B antibody [C2C3], C-term (GTX116198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KDM3B antibody [C2C3], C-term
GTX116198
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetKDM3B
Overview
- SupplierGeneTex
- Product NameKDM3B antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID51780
- Target nameKDM3B
- Target descriptionlysine demethylase 3B
- Target synonyms5qNCA, C5orf7, DIJOS, JMJD1B, NET22, lysine-specific demethylase 3B, [histone H3]-dimethyl-L-lysine(9) demethylase 3B, jmjC domain-containing histone demethylation protein 2B, jumonji domain containing 1B, jumonji domain-containing protein 1B, lysine (K)-specific demethylase 3B, nuclear protein 5qNCA
- HostRabbit
- IsotypeIgG
- Protein IDQ7LBC6
- Protein NameLysine-specific demethylase 3B
- Scientific DescriptionHistone demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- D'Amore C, Borgo C, Bosello Travain V, et al. KDM2A and KDM3B as Potential Targets for the Rescue of F508del-CFTR. Int J Mol Sci. 2022,23(17). doi: 10.3390/ijms23179612Read this paper
Datasheet
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