ICC/IF analysis of HeLa cells using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8]. Dilution : 1:2000 Fixation : 4% PFA, overnight Permeabilization : 0.25% Triton X-100 in PBS for 10 min
![WB analysis of various samples using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8]. Left panel : SDS-PAGE with CBB staining Right panel : Primary antibody Lane 1 : HeLa cells transfected control siRNA Lane 2 : HeLa cells transfected RBP2 siRNA Lane 3 : MCF-7 whole cell lysate Lane 4 : U2OS whole cell lysate Lane 5 : NIH3T3 whole cell lysate Lane 6 : J1 (mouse ES cells) whole cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX00688/GTX00688_20191104_WB_w_23053121_595.webp "WB analysis of various samples using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8]. Left panel : SDS-PAGE with CBB staining Right panel : Primary antibody Lane 1 : HeLa cells transfected control siRNA Lane 2 : HeLa cells transfected RBP2 siRNA Lane 3 : MCF-7 whole cell lysate Lane 4 : U2OS whole cell lysate Lane 5 : NIH3T3 whole cell lysate Lane 6 : J1 (mouse ES cells) whole cell lysate")
ICC/IF analysis of HeLa cells using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8]. Dilution : 1:2000 Fixation : 4% PFA, overnight Permeabilization : 0.25% Triton X-100 in PBS for 10 min
KDM5A / Jarid1A / RBBP2 antibody [18E8]
GTX00688
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetKDM5A
Overview
- SupplierGeneTex
- Product NameKDM5A / Jarid1A / RBBP2 antibody [18E8] - KO/KD Validated
- Delivery Days Customer9
- Application Supplier NoteWB: ~1 microg/ml. ICC/IF: ~1 microg/ml. FACS: using 1 microg for 106 cells.. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID18E8
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID5927
- Target nameKDM5A
- Target descriptionlysine demethylase 5A
- Target synonyms[histone H3]-trimethyl-L-lysine(4) demethylase 5A; histone demethylase JARID1A; Jumonji, AT rich interactive domain 1A (RBP2-like); jumonji/ARID domain-containing protein 1A; lysine (K)-specific demethylase 5A; lysine-specific demethylase 5A; RBBP2; RBBP-2; RBP2; retinoblastoma binding protein 2
- HostMouse
- IsotypeIgG2a
- Protein IDP29375
- Protein NameLysine-specific demethylase 5A
- Scientific DescriptionThis gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex. Nishibuchi G et al., 2014 Oct 17, J Biol ChemRead more