
293T whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM5A / Jarid1A antibody (GTX130278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KDM5A / Jarid1A / RBBP2 antibody
GTX130278
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetKDM5A
Overview
- SupplierGeneTex
- Product NameKDM5A / Jarid1A / RBBP2 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration2.11 mg/ml
- ConjugateUnconjugated
- Gene ID5927
- Target nameKDM5A
- Target descriptionlysine demethylase 5A
- Target synonymsNEDEHC, RBBP-2, RBBP2, RBP2, lysine-specific demethylase 5A, Jumonji, AT rich interactive domain 1A (RBP2-like), [histone H3]-trimethyl-L-lysine(4) demethylase 5A, histone demethylase JARID1A, jumonji/ARID domain-containing protein 1A, lysine (K)-specific demethylase 5A, retinoblastoma binding protein 2
- HostRabbit
- IsotypeIgG
- Protein IDP29375
- Protein NameLysine-specific demethylase 5A
- Scientific DescriptionThe protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161





![WB analysis of various samples using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8]. Left panel : SDS-PAGE with CBB staining Right panel : Primary antibody Lane 1 : HeLa cells transfected control siRNA Lane 2 : HeLa cells transfected RBP2 siRNA Lane 3 : MCF-7 whole cell lysate Lane 4 : U2OS whole cell lysate Lane 5 : NIH3T3 whole cell lysate Lane 6 : J1 (mouse ES cells) whole cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX00688/GTX00688_20191104_WB_w_23053121_595.webp)
![WB analysis of various samples using GTX01204 KDM5A / Jarid1A / RBBP2 antibody [9A6] (right). Left : SDS-PAGE with CBB staining Lane 1 : HeLa control siRNA Lane 2 : HeLa RBP2 siRNA Lane 3 : MCF-7 whole cell lysate Lane 4 : U2OS whole cell lysate Lane 5 : NIH-3T3 whole cell lysate Lane 6 : J1 (mouse ES) whole cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX01204/GTX01204_20200406_AP_001_130_w_23053121_218.webp)