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Western blot analysis of extracts of various cell lines using KHDRBS1 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using KHDRBS1 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using KHDRBS1 Polyclonal Antibody at dilution of 1:1000.

KHDRBS1 Polyclonal Antibody

E-AB-62359
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    KHDRBS1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sam68/KHDRBS1 antigen affinity purified polyclonal antibody (Catalog # A01717-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sam68/KHDRBS1 at approximately 68 kDa. The expected band size for Sam68/KHDRBS1 is at 48 kDa.
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