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Western blot analysis of extracts of various cell lines using KIAA1524 Polyclonal Antibody at 1:3000 dilution.
Western blot analysis of extracts of various cell lines using KIAA1524 Polyclonal Antibody at 1:3000 dilution.
Western blot analysis of extracts of various cell lines using KIAA1524 Polyclonal Antibody at 1:3000 dilution.

KIAA1524 Polyclonal Antibody

E-AB-63677
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    KIAA1524 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KIAA1524 using anti-KIAA1524 antibody (PB9714). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Mouse Testis Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIAA1524 antigen affinity purified polyclonal antibody (Catalog # PB9714) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIAA1524 at approximately 90 kDa. The expected band size for KIAA1524 is at 102 kDa.
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