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ProductsBack/KIAA1754L / ITPRIPL1 Antibody (aa402-451)

KIAA1754L / ITPRIPL1 Antibody (aa402-451)

LS-C40485
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetITPRIPL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KIAA1754L / ITPRIPL1 Antibody (aa402-451)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.2 - 1 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID150771
  • Target name
    ITPRIPL1
  • Target description
    ITPRIP like 1
  • Target synonyms
    D1B, KIAA1754L, inositol 1,4,5-trisphosphate receptor-interacting protein-like 1, DANGER family member 1B, inositol 1,4,5-triphosphate receptor-interacting protein-like 1, inositol 1,4,5-trisphosphate receptor interacting protein-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ITPRIPL1 using anti-ITPRIPL1 antibody (A17509-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPRIPL1 antigen affinity purified polyclonal antibody (Catalog # A17509-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPRIPL1 at approximately 70 kDa. The expected band size for ITPRIPL1 is at 63 kDa.
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ReactivityHuman
TargetITPRIPL1
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The Binding Activity of Macaca fascicularis ITPRIPL1 with Anti-ITPRIPL1 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ITPRIPL1 (CSB-MP6125MOV) at 2 microg/mL can bind Anti-ITPRIPL1 recombinant antibody. The EC50 is 3.759-4.428 ng/mL.
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ReactivityHuman, Monkey
TargetITPRIPL1
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ReactivityHuman
TargetITPRIPL1
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Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE GTX120868 diluted at 1:1000
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ITPRIPL1 antibody [C1C3]GTX120868
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ReactivityHuman
TargetITPRIPL1
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