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Western blot All lanes: KIF3A antibody at 9.45ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Rat gonad tissue Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa
Western blot All lanes: KIF3A antibody at 9.45ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Rat gonad tissue Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa
Western blot All lanes: KIF3A antibody at 9.45ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Rat gonad tissue Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa

KIF3A Antibody

CSB-PA012335ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetKIF3A
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Overview

  • Supplier
    Cusabio
  • Product Name
    KIF3A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11127
  • Target name
    KIF3A
  • Target description
    kinesin family member 3A
  • Target synonyms
    FLA10, KLP-20, kinesin-like protein KIF3A, kinesin family protein 3A, microtubule plus end-directed kinesin motor 3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y496
  • Protein Name
    Kinesin-like protein KIF3A
  • Scientific Description
    Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation (By similarity).
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KIF3A using anti-KIF3A antibody (PB9654). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Mouse Brain Tissue Lysate at 50ug, Lane 4: Mouse Testis Tissue Lysate at 50ug, Lane 5: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF3A antigen affinity purified polyclonal antibody (Catalog # PB9654) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF3A at approximately 80 kDa. The expected band size for KIF3A is at 80 kDa.
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