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Western Blot analysis of COLO205 cells using KIF4A Polyclonal Antibody
Western Blot analysis of COLO205 cells using KIF4A Polyclonal Antibody
Western Blot analysis of COLO205 cells using KIF4A Polyclonal Antibody

KIF4A Antibody

CSB-PA003098
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetKIF4A
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Overview

  • Supplier
    Cusabio
  • Product Name
    KIF4A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID24137
  • Target name
    KIF4A
  • Target description
    kinesin family member 4A
  • Target synonyms
    KIF4, KIF4G1, MRX100, TMDI, XLID100, chromosome-associated kinesin KIF4A, chromokinesin KIF4A, chromokinesin-A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95239
  • Protein Name
    Chromosome-associated kinesin KIF4A
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KIF4A/4B using anti-KIF4A/4B antibody (A04486-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF4A/4B antigen affinity purified polyclonal antibody (Catalog # A04486-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF4A/4B at approximately 140 kDa. The expected band size for KIF4A/4B is at 140 kDa.
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