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KIF4A Antibody (YA2653, Rabbit)

HY-P82908
MedChem Express
TargetKIF4A
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    KIF4A Antibody (YA2653, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID24137
  • Target name
    KIF4A
  • Target description
    kinesin family member 4A
  • Target synonyms
    KIF4, KIF4G1, MRX100, TMDI, XLID100, chromosome-associated kinesin KIF4A, chromokinesin KIF4A, chromokinesin-A
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    KIF4A Antibody (YA2653) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2653), targeting KIF4A, with a predicted molecular weight of 140 kDa (observed band size: 150 kDa). KIF4A Antibody (YA2653) can be used for WB, FC experiment in human, mouse background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KIF4A/4B using anti-KIF4A/4B antibody (A04486-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF4A/4B antigen affinity purified polyclonal antibody (Catalog # A04486-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF4A/4B at approximately 140 kDa. The expected band size for KIF4A/4B is at 140 kDa.
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