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ProductsBack/Kindlin 2 (FERMT2) Mouse Monoclonal Antibody [Clone ID: OTI9E4]
Simple Western™ analysis of endogenous protein FERMT2 from HepG2 lysates (0.2 mg/mL) using FERMT2 Mouse Monoclonal Antibody #TA500504. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Simple Western™ analysis of endogenous protein FERMT2 from HepG2 lysates (0.2 mg/mL) using FERMT2 Mouse Monoclonal Antibody #TA500504. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Simple Western™ analysis of endogenous protein FERMT2 from HepG2 lysates (0.2 mg/mL) using FERMT2 Mouse Monoclonal Antibody #TA500504. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Kindlin 2 (FERMT2) Mouse Monoclonal Antibody [Clone ID: OTI9E4]

CF500504
OriGene
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetFERMT2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Kindlin 2 (FERMT2) Mouse Monoclonal Antibody [Clone ID: OTI9E4]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI9E4
  • Gene ID10979
  • Target name
    FERMT2
  • Target description
    FERM domain containing kindlin 2
  • Target synonyms
    KIND2, MIG2, PLEKHC1, UNC112, UNC112B, mig-2, fermitin family homolog 2, PH domain-containing family C member 1, fermitin family member 2, kindlin 2, mitogen inducible gene 2 protein, mitogen inducible gene-2, pleckstrin homology domain containing, family C (with FERM domain) member 1, pleckstrin homology domain containing, family C member 1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ96AC1
  • Protein Name
    Fermitin family homolog 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) FERMT2 mouse monoclonal antibody, clone OTI9E4 (formerly 9E4)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Kindlin 2/FERMT2 using anti-Kindlin 2/FERMT2 antibody (A03653-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kindlin 2/FERMT2 antigen affinity purified polyclonal antibody (Catalog # A03653-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kindlin 2/FERMT2 at approximately 70 kDa. The expected band size for Kindlin 2/FERMT2 is at 70 kDa.
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