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Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: KIR3DL1 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 39 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: KIR3DL1 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 39 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: KIR3DL1 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 39 kDa Observed band size: 50 kDa

KIR3DL1 Antibody

CSB-PA012364LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetKIR3DL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KIR3DL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3811
  • Target name
    KIR3DL1
  • Target description
    killer cell immunoglobulin like receptor, three Ig domains and long cytoplasmic tail 1
  • Target synonyms
    CD158 antigen-like family member E; CD158E1; HLA-BW4-specific inhibitory NK cell receptor; killer cell immunoglobulin-like receptor 3DL1; killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1; KIR; KIR antigen 3DL1; KIR2DL5B; KIR3DL1/S1; natural killer-associated transcript 3; NKAT3; NKAT-3; NKB1; NKB1B; p70 killer cell inhibitory receptor; p70 natural killer cell receptor clones CL-2/CL-11; p70 NK receptor CL-2/CL-11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43629
  • Protein Name
    Killer cell immunoglobulin-like receptor 3DL1
  • Scientific Description
    Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KIR3DL1 using anti-KIR3DL1 antibody (A02187-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIR3DL1 antigen affinity purified polyclonal antibody (Catalog # A02187-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIR3DL1 at approximately 49 kDa. The expected band size for KIR3DL1 is at 49 kDa.
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