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ProductsBack/KITLG Antibody
Western blot All lanes: Kit ligand antibody at 4ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 31, 28, 27 kDa Observed band size: 31 kDa
Western blot All lanes: Kit ligand antibody at 4ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 31, 28, 27 kDa Observed band size: 31 kDa
Western blot All lanes: Kit ligand antibody at 4ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 31, 28, 27 kDa Observed band size: 31 kDa

KITLG Antibody

CSB-PA012376ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetKITLG
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Overview

  • Supplier
    Cusabio
  • Product Name
    KITLG Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4254
  • Target name
    KITLG
  • Target description
    KIT ligand
  • Target synonyms
    DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SCF, SF, SHEP7, SLF, WS2F, kit ligand, c-Kit ligand, familial progressive hyperpigmentation 2, mast cell growth factor, steel factor, stem cell factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21583
  • Protein Name
    Kit ligand
  • Scientific Description
    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SCF using anti-SCF antibody (A01254-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCF antigen affinity purified polyclonal antibody (Catalog # A01254-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCF at approximately 37KD. The expected band size for SCF is at 31KD.
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Mouse spleen cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:33 for 30 min at room temperature. A Goat anti-rabbit IgG-AF647 secondary antibody was used at 1ug for 40 min at room temperature. Acquisition of 10,000 events was performed. Primary antibody staining (green) is compared to compared to unstained cells (purple), secondary only (light blue), and isotype control (orange).
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