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KITLG Antibody

HY-P84178
MedChem Express
TargetKITLG
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    KITLG Antibody
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4254
  • Target name
    KITLG
  • Target description
    KIT ligand
  • Target synonyms
    c-Kit ligand; DCUA; DFNA69; familial progressive hyperpigmentation 2; FPH2; FPHH; kit ligand; Kitl; KL-1; mast cell growth factor; MGF; SCF; SF; SHEP7; SLF; steel factor; stem cell factor
  • Host
    Mouse
  • Isotype
    IgG2b
  • UNSPSC
    12352203

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Mouse spleen cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:33 for 30 min at room temperature. A Goat anti-rabbit IgG-AF647 secondary antibody was used at 1ug for 40 min at room temperature. Acquisition of 10,000 events was performed. Primary antibody staining (green) is compared to compared to unstained cells (purple), secondary only (light blue), and isotype control (orange).
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Figure 1. Western blot analysis of SCF using anti-SCF antibody (A01254-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCF antigen affinity purified polyclonal antibody (Catalog # A01254-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCF at approximately 37KD. The expected band size for SCF is at 31KD.
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