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WB analysis of HepG2 nuclear (A) and HeLa (B) cell lysate using GTX89919 KLF15 antibody, N-term. Dilution : 1μg/ml Loading : 35μg protein in RIPA buffer
WB analysis of HepG2 nuclear (A) and HeLa (B) cell lysate using GTX89919 KLF15 antibody, N-term. Dilution : 1μg/ml Loading : 35μg protein in RIPA buffer
WB analysis of HepG2 nuclear (A) and HeLa (B) cell lysate using GTX89919 KLF15 antibody, N-term. Dilution : 1μg/ml Loading : 35μg protein in RIPA buffer

KLF15 antibody, N-term

GTX89919
GeneTex
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetKLF15
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Overview

  • Supplier
    GeneTex
  • Product Name
    KLF15 antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.3-1microg/ml. FCM: 10microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID28999
  • Target name
    KLF15
  • Target description
    KLF transcription factor 15
  • Target synonyms
    KKLF, Krueppel-like factor 15, Kruppel like factor 15, kidney-enriched Kruppel-like factor, kidney-enriched krueppel-like factor
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9UIH9
  • Protein Name
    Krueppel-like factor 15
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KLF15 using anti-KLF15 antibody (A03661-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF15 antigen affinity purified polyclonal antibody (Catalog # A03661-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF15 at approximately 44 kDa. The expected band size for KLF15 is at 44 kDa.
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