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KLF4 antibody

GTX101509
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKLF4
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Overview

  • Supplier
    GeneTex
  • Product Name
    KLF4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9314
  • Target name
    KLF4
  • Target description
    KLF transcription factor 4
  • Target synonyms
    EZF, GKLF, Krueppel-like factor 4, Kruppel like factor 4, Kruppel-like factor 4 (gut), endothelial Kruppel-like zinc finger protein, epithelial zinc finger protein EZF, gut Kruppel-like factor, gut-enriched krueppel-like factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43474
  • Protein Name
    Krueppel-like factor 4
  • Scientific Description
    Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Feng DD, Zheng B, Yu J, et al. 17β-Estradiol Inhibits Proliferation and Oxidative Stress in Vascular Smooth Muscle Cells by Upregulating BHLHE40 Expression. Front Cardiovasc Med. 2021,8:768662. doi: 10.3389/fcvm.2021.768662
    Read this paper
  • Zhou J, Zhang L, Zheng B, et al. Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose. J Ethnopharmacol. 2020,262:113208. doi: 10.1016/j.jep.2020.113208
    Read this paper
  • Nakayama I, Higa-Nakamine S, Uehara A, et al. Regulation of epidermal growth factor receptor expression and morphology of lung epithelial cells by interleukin-1β. J Biochem. 2020,168(2):113-123. doi: 10.1093/jb/mvaa015
    Read this paper
  • Takeuchi Y, Kito A, Itoh S, et al. Kruppel-Like Factor 4 represses osteoblast differentiation via ciliary Hedgehog signaling. Exp Cell Res. 2018,371(2):417-425. doi: 10.1016/j.yexcr.2018.09.002
    Read this paper
  • Moon BS, Bai J, Cai M, et al. Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis. Nat Commun. 2018,9(1):401. doi: 10.1038/s41467-017-02720-9
    Read this paper
  • Guo Y, Yu Q, Mathew S, et al. Cocktail of Chemical Compounds and Recombinant Proteins Robustly Promote the Stemness of Adipose-Derived Stem Cells. Cell Reprogram. 2017,19(6):363-371. doi: 10.1089/cell.2017.0022
    Read this paper
  • Ducarouge B, Pelissier-Rota M, Powell R, et al. Involvement of CRF2 signaling in enterocyte differentiation. World J Gastroenterol. 2017,23(28):5127-5145. doi: 10.3748/wjg.v23.i28.5127
    Read this paper

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Figure 1. Western blot analysis of KLF4 using anti-KLF4 antibody (A00120-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF4 antigen affinity purified polyclonal antibody (Catalog # A00120-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF4 at approximately 60 kDa. The expected band size for KLF4 is at 60 kDa.
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