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Western blot All lanes: KMO antibody at 10ug/ml + Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 55, 52 kDa Observed band size: 56 kDa
Western blot All lanes: KMO antibody at 10ug/ml + Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 55, 52 kDa Observed band size: 56 kDa
Western blot All lanes: KMO antibody at 10ug/ml + Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 55, 52 kDa Observed band size: 56 kDa

KMO Antibody

CSB-PA012475ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetKMO
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Overview

  • Supplier
    Cusabio
  • Product Name
    KMO Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8564
  • Target name
    KMO
  • Target description
    kynurenine 3-monooxygenase
  • Target synonyms
    dJ317G22.1; kynurenine 3-hydroxylase; kynurenine 3-monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15229
  • Protein Name
    Kynurenine 3-monooxygenase
  • Scientific Description
    Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KMO using anti-KMO antibody (A05469-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMO antigen affinity purified polyclonal antibody (Catalog # A05469-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KMO at approximately 50 kDa. The expected band size for KMO is at 56 kDa.
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