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WB analysis of mouse liver lysate using GTX81111 KMO antibody, Internal. Loading : 20 microg per lane Dilution : 1:2000
WB analysis of mouse liver lysate using GTX81111 KMO antibody, Internal. Loading : 20 microg per lane Dilution : 1:2000
WB analysis of mouse liver lysate using GTX81111 KMO antibody, Internal. Loading : 20 microg per lane Dilution : 1:2000

KMO antibody, Internal

GTX81111
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetKMO
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Overview

  • Supplier
    GeneTex
  • Product Name
    KMO antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. IHC-P: 1:10-1:50. FACS: 1:10-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8564
  • Target name
    KMO
  • Target description
    kynurenine 3-monooxygenase
  • Target synonyms
    dJ317G22.1; kynurenine 3-hydroxylase; kynurenine 3-monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15229
  • Protein Name
    Kynurenine 3-monooxygenase
  • Scientific Description
    This gene encodes a mitochondrion outer membrane protein that catalyzes the hydroxylation of L-tryptophan metabolite, L-kynurenine, to form L-3-hydroxykynurenine. Studies in yeast identified this gene as a therapeutic target for Huntington disease. [provided by RefSeq, Oct 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KMO using anti-KMO antibody (A05469-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMO antigen affinity purified polyclonal antibody (Catalog # A05469-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KMO at approximately 50 kDa. The expected band size for KMO is at 56 kDa.
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