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KMT1B Antibody (YA1882)

HY-P82137
MedChem Express
TargetSUV39H2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    KMT1B Antibody (YA1882)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID79723
  • Target name
    SUV39H2
  • Target description
    SUV39H2 histone lysine methyltransferase
  • Target synonyms
    H3-K9-HMTase 2; histone H3-K9 methyltransferase 2; histone methyltransferase SUV39H2; histone-lysine N-methyltransferase SUV39H2; KMT1B; lysine N-methyltransferase 1B; su(var)3-9 homolog 2; suppressor of variegation 3-9 homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    KMT1B Antibody (YA1882) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1882), targeting KMT1B, with a predicted molecular weight of 47 kDa (observed band size: 47 kDa). KMT1B Antibody (YA1882) can be used for WB, IHC-P, IP experiment in human, mouse, rat background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KMT1B/SUV39H2 using anti-KMT1B/SUV39H2 antibody (A05361-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMT1B/SUV39H2 antigen affinity purified polyclonal antibody (Catalog # A05361-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KMT1B/SUV39H2 at approximately 47 kDa. The expected band size for KMT1B/SUV39H2 is at 47 kDa.
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